Glossary entry (derived from question below)
German term or phrase:
Durchfixierung
English translation:
complete fixation
Added to glossary by
Rowan Morrell
Sep 20, 2012 05:59
11 yrs ago
German term
New Zealand
Committed to the Customer
Native in: English (Variant: New Zealand) 
Works in: French to English, German to English, Spanish to English
Durchfixierung
German to English
Medical
Medical: Pharmaceuticals
Laboratory Experiments on Rats
"Histologische Untersuchung bei Versuchsende
Nach Durchfixierung wurden die Organe zurechtgeschnitten oder Proben entnommen und im Entwässerungsautomaten weiterbearbeitet."
From a text about an experiment on lab rats. This is describing what happens after they're put down. The text has previously mentioned about organs being "fixiert" in formalin, which I guess is just fixed, but I have no idea what "Durchfixierung" is and can't find a translation for it (or the verb "durchfixieren" anywhere). Google searching for "fixed through" in relation to animal organs in experiments is utterly inconclusive. TIA for any light you can shed on this decidedly obscure word.
Nach Durchfixierung wurden die Organe zurechtgeschnitten oder Proben entnommen und im Entwässerungsautomaten weiterbearbeitet."
From a text about an experiment on lab rats. This is describing what happens after they're put down. The text has previously mentioned about organs being "fixiert" in formalin, which I guess is just fixed, but I have no idea what "Durchfixierung" is and can't find a translation for it (or the verb "durchfixieren" anywhere). Google searching for "fixed through" in relation to animal organs in experiments is utterly inconclusive. TIA for any light you can shed on this decidedly obscure word.
Proposed translations
(English)
| 4 +5 | complete fixation |
SJLD
|
| 2 | immersion fixation |
Zareh Darakjian Ph.D.
|
Proposed translations
+5
13 mins
Selected
complete fixation
Once fixation is complete...
Effects of Prolonged Formalin Fixation on Diagnostic ...
www.ncbi.nlm.nih.gov › ... › J Histochem Cytochem › v.57(8); Aug 2009
by JD Webster - 2009 - Cited by 13 - Related articles
It takes ~25 hr for formalin to penetrate a 1-cm3 sphere of tissue (Eltoum et al. 2001b). If tissues are processed before complete fixation, unfixed tissue will be ...
[PDF]
Fixation and Fixatives (4) – Popular Fixative Solutions: Pathology ...
www.leica-microsystems.com/.../fixation-and-fixatives-4-pop...
6 Mar 2012 – Combines a denaturing fixative with the additive and cross-linking effects of formalin. Is sometimes used during processing to complete fixation ...
Guidelines for Transport, Handling, and Fixation of Tissues
pathology.wustl.edu/research/cores/amp/index.php?page...
After complete fixation in formalin, tissues can be safely transferred to 70% ethanol at 4o C until processing. As implied above, under-fixation should be avoided.
Effects of Prolonged Formalin Fixation on Diagnostic ...
www.ncbi.nlm.nih.gov › ... › J Histochem Cytochem › v.57(8); Aug 2009
by JD Webster - 2009 - Cited by 13 - Related articles
It takes ~25 hr for formalin to penetrate a 1-cm3 sphere of tissue (Eltoum et al. 2001b). If tissues are processed before complete fixation, unfixed tissue will be ...
[PDF]
Fixation and Fixatives (4) – Popular Fixative Solutions: Pathology ...
www.leica-microsystems.com/.../fixation-and-fixatives-4-pop...
6 Mar 2012 – Combines a denaturing fixative with the additive and cross-linking effects of formalin. Is sometimes used during processing to complete fixation ...
Guidelines for Transport, Handling, and Fixation of Tissues
pathology.wustl.edu/research/cores/amp/index.php?page...
After complete fixation in formalin, tissues can be safely transferred to 70% ethanol at 4o C until processing. As implied above, under-fixation should be avoided.
Peer comment(s):
| agree |
Zareh Darakjian Ph.D.
: It would seem to me that this is the correct translation for the term in question.
44 mins
|
|
thanks Dr Z! :-)
|
|
| agree |
Dr Lofthouse
1 hr
|
| agree |
Anne Schulz
: complete as "in toto", I suppose (since rat organs are small enough to fit into a cassette)
9 hrs
|
| agree |
uyuni
10 hrs
|
| agree |
Harald Moelzer (medical-translator)
1 day 58 mins
|
4 KudoZ points awarded for this answer.
Comment: "Complete fixation it is! Thanks for a thorough and convincing answer SJLD, and thanks to the peer graders for making my decision that much easier. But thanks also to Zareh for setting me straight about fixation. Appreciate everyone's help."
21 mins
Zareh Darakjian Ph.D.
United States
United States
Native in English
Specializes in field
United States
A scientist, pianist and a translator
Native in: English 
, Armenian
Works in: German to English, French to English, Spanish to English, and 2 more.
Related KudoZ points
:
112
immersion fixation
This could be "immersion fixation", the other two major types being
"heat fixation" and "immersion".
http://en.wikipedia.org/wiki/Fixation_(histology)#Types_of_f...
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Note added at 23 mins (2012-09-20 06:22:42 GMT)
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http://en.wikipedia.org/wiki/Fixation_(histology)
--------------------------------------------------
Note added at 24 mins (2012-09-20 06:23:33 GMT)
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**Immersion**: The sample of tissue is immersed in fixative of volume at a minimum of 20 times greater than the volume of the tissue to be fixed. The fixative must diffuse through the tissue to fix, so tissue size and density, as well as type of fixative must be considered. Using a larger sample means it takes longer for the fixative to reach the deeper tissue. Best in a slight vacuum.
"heat fixation" and "immersion".
http://en.wikipedia.org/wiki/Fixation_(histology)#Types_of_f...
--------------------------------------------------
Note added at 23 mins (2012-09-20 06:22:42 GMT)
--------------------------------------------------
http://en.wikipedia.org/wiki/Fixation_(histology)
--------------------------------------------------
Note added at 24 mins (2012-09-20 06:23:33 GMT)
--------------------------------------------------
**Immersion**: The sample of tissue is immersed in fixative of volume at a minimum of 20 times greater than the volume of the tissue to be fixed. The fixative must diffuse through the tissue to fix, so tissue size and density, as well as type of fixative must be considered. Using a larger sample means it takes longer for the fixative to reach the deeper tissue. Best in a slight vacuum.
Discussion